A New Strategy of Using O18-Labeled Iodoacetic Acid for Mass Spectrometry-Based Protein Quantitation
نویسندگان
چکیده
منابع مشابه
A new strategy of using O18-labeled iodoacetic acid for mass spectrometry-based protein quantitation.
A new O(18) labeling protocol is designed to assist quantitation of cysteine-containing proteins using LC/MS. Unlike other O(18) labeling strategies, the labeling is carried out at the intact protein level (prior to its digestion) during reduction/alkylation of cysteine side chains using O(18)-labeled iodoacetic acid (IAA). The latter can be easily prepared by exchanging carboxylic oxygen atoms...
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ژورنال
عنوان ژورنال: Journal of The American Society for Mass Spectrometry
سال: 2012
ISSN: 1044-0305,1879-1123
DOI: 10.1007/s13361-012-0396-9